HPLC Columns - Pursuit Columns
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KB005936: Specifications and Options for Pursuit LC Columns
 
C18
Agilent Pursuit C18 columns feature a C18 phase covalently bonded to special 200Å ultra-pure silica.
 
Particle Size (µm) Length (mm) ID Specifications Method Development Notes Working with LC/MS
3
5
10
50-250   Endcapped
Pore size: 200Å
Surface area: 200 m2/g
pH: 2.0 - 9.0
Carbon load: 12.9%
Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
 
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