What is the effect on an LC analysis of changing the column diameter?
 
   If column diameter halves, sensitivity increaces by factor of four to five (assuming the injection volume is kept constant). For example ,when a sample is injected onto a 2.1mm id column, the peaks are about a factor five higher than when the same amount of sample is injected onto a 4.6 mm id column.

   As long as the linear flow is maintained, parameters such as column efficiency, theoretical plates, back pressure and analysis time are unaffected by column ID reduction
 
 
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