How do I prevent peak tailing and splitting?
Column related causes:  
 
Cause

Action

Column fouling

Clean or replace column

Particulates on column

Reverse flush column

Replace inlet frit

Column void or channel

Replace column or fill void

"Worn-out" guard column

Replace guard column

Bad sample distribution

Change to a column with a better inlet design

Non column related causes:

Excessive extra-column volume), sample overload, changes in mobile phase composition
(additives, pH, buffer concentration).

 
 
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