What is the effect on the LC separation of changing the pore size?
 
      Smaller pore size allows higher linear velocity of mobiler phase. The optimum mobile phase linear velocity is determined by the particle size of the stationary phase. At the optimum linear velocity, the column generates the maximum number of theoretical plates. For columns with 4.6mm id, plate numbers are maximized at about 0.75 ml/min for 10um particles or 1.5 ml/min for 5um particles.
 
 
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