What can cause a loss in column efficiency (theoretical plates)?

Column related causes:

Cause

Action

Column developed a void

Fill in the void with similar packing material and reverse or replace the column

"Worn-out" guard column

Replace guard column

 

Other possible causes:

Excessive extra-column volume, injected sample volume too large, strength of  sample  solvent  stronger than mobile phase, changes in mobile phase composition

 
 
  Copyright © LCGC 2013-2014. All Right Reserved.