HPLC - Poroshell 120 Columns
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KB005935: Agilent Poroshell 120 Column Specifications and Options
 
Poroshell 120 EC-C18
The EC-C18 bonded phase is made by first chemically bonding a dense monolayer of dimethyl-n-octadecyl silane stationary phase to the porous shell of the Poroshell 120 silica support. The bonded phase packing is then endcapped using proprietary reagents and procedures to obtain maximum deactivtion of the silica surface. The exhaustive endcapping makes it ideal for use with basic compounds, especially those that produce poor peak shapes on other columns. Poroshell 120 EC-C18 performs very similarly to ZORBAX Eclipse Plus C18 and can usually be easily transferred to and from methods using ZORBAX Eclipse Plus C18. It is an exceptional first choice for most separations, including peptide mapping with LC/MS compatible mobile phases.
 
Particle Size (µm) Length (mm) ID Specifications Method Development Notes Working with LC/MS
4.0 and 2.7 µm superficially porous particles, comprised of solid core and porous outer shell. 30
50
75
100
150
2.1
3.0
4.6
Endcapped;
Pore size: 120Å
Surface area: 130 m2/g
pH: 2.0 - 8.0
Max temperature: 60 °C
Max pressure: 600 bar
Carbon load: 8%
Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
 
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