HPLC - Poroshell 120 Columns
KB005935: Agilent Poroshell 120 Column Specifications and Options
Poroshell 120 EC-C8
An EC-C8 bonded phase is applied to the totally porous outer layer. It will be less retentive for non-polar samples than Poroshell 120 EC-C18. The EC-C8 bonded phase is made by first chemically bonding a dense monolayer of dimethyl-n-octyl silane stationary phase to the porous shell of the Poroshell 120 silica support. The bonded phase packing is then endcapped using proprietary reagents and procedures to obtain maximum deactivation of the silica surface. The exhaustive endcapping makes it ideal for use with basic compounds, especially those that produce poor peak shapes on other columns. These columns can be used for a wide range of applications.
Particle Size (µm) Length (mm) ID Specifications Method Development Notes Working with LC/MS
4.0 and 2.7 µm superficially porous particles, comprised of solid core and porous outer shell. 30
Pore size: 120Å
Surface area: 130 m2/g
pH: 2.0 - 8.0
Max temperature: 60 °C
Max pressure: 600 bar
Carbon load: 5%
Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
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