HPLC - Poroshell 120 Columns
KB005935: Agilent Poroshell 120 Column Specifications and Options
Poroshell 120 PFP
The Agilent Poroshell 120 PFP provides another unique phase chemistry on superficially porous particles. The pentafluorophenyl (PFP) end group provides excellent separation of polar and halogenated analytes, as well as powerful selectivity towards aromatic groups due to π-electron interactions.
Particle Size (µm) Length (mm) ID Specifications Method Development Notes Working with LC/MS
4.0 and 2.7 µm superficially porous particles, comprised of solid core and porous outer shell. 50
Pore size: 120Å
Surface area: 130 m2/g
pH: 2.0 - 9.0
Max temperature: 60 °C
Max pressure: 600 bar
Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
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