HPLC - Poroshell 120 Columns
KB005935: Agilent Poroshell 120 Column Specifications and Options
Porshell 120 StableBond SB-C18
The StableBond SB-C18 bonded phase is made by chemically bonding a sterically-protected C18 stationary phase to the porous shell of the Poroshell 120 silica support. The densely covered, sterically protected, diisobutyl-n-octadecylsilane stationary phase is chemically stable and gives long column life at low pH (pH 1- 2). The Agilent Poroshell 120 SB-C18 column is not endcapped, which provides greater alternative selectivity. It can be used for basic, neutral or acidic samples and is particularly well suited for use with aggressive low pH mobile phases (for example, pH <2, high ionic strength (>25 mM), ion-pair additives, etc.) since the steric protection of the bonded phase resists degradation with such mobile phases.
Particle Size (µm) Length (mm) ID Specifications Method Development Notes Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell. 50
Pore size: 120Å
Surface area: 130 m2/g
pH: 1.0 - 8.0
Max temperature: 60 °C
Max pressure: 600 bar
Carbon load: 7.5%
Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
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