Bio Columns
Poroshell 300
Agilent Poroshell columns are ideal for fast separations of proteins and peptides because the unique particle allows for fast flow rates to be used while maintaining sharp, efficient peaks. Peptides and proteins are typically separated slowly to reduce the potential peak broadening of these slow diffusing analytes. But Poroshell columns use a unique particle made with a layer of porous silica on a solid core of silica. This reduces the diffusion distance for proteins making practical rapid HPLC separations of peptides and proteins up to 500 – 1,000 kDA. Poroshell 300SB C18, C8, C3, and 300Extend columns provide excellent stability and selectivity choices with TFA and formic acid mobile faces. Poroshell 300Extend C18 columns can be used at varying pH levels for unique separations and additional selectivity choices.
- UHPLC separations of biomolecules with superficially porous particles
- 300Å pore provide high efficiency and recovery with proteins (up to 1,000 kDa) and monoclonal antibodies
- Achieve long lifetime at low pH with Poroshell 300SB; at high pH with 300Extend-C18
- Optimize recovery and selectivity with four different bonded phases – 300SB-C18, 300SB-C8, 300SB-C3, and 300Extend-C18
Bonded Phase Pore Size Temp Limits* pH Range Endcapped USP Designation
Poroshell 300SB-C18 300Å 90 ºC 1.0-8.0 No L1
Poroshell 300SB-C8 300Å 90 ºC 1.0-8.0 No L7
Poroshell 300SB-C3 300Å 90 ºC 1.0-8.0 No L56
Poroshell 300Extend-C18 300Å 40 ºC above pH 8 60 ºC below pH 8 2.0-11.0 Yes L1
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