HPLC Columns - Zorbax Columns
Agilent ZORBAX Eclipse Plus Column Specifications and Options
ZORBAX Eclipse Plus C18
The Agilent ZORBAX Eclipse Plus C18 packing is made by bonding a dense monolayer of dimethyl-n-octadecylsilane (C18) stationary phase to special Eclipse Plus silica. Eclipse Plus C18 is an excellent choice for method development because of its exceptional performance and peak shapes for acids, bases and neutrals. Poroshell 120 EC-C18 is very similar to ZORBAX Eclipse Plus C18.
Particle Size (µm) Length (mm) ID Specifications Method Development Notes Working with LC/MS
1.8 µm particles (both RRHD, stable to 1200 bar, and RRHT,
stable to 600 bar)
3.5 µm
5 µm
20 - 250 2.1
Custom options available
Pore size: 95Å
Surface area: 180 m2/g
pH: 2.0 - 9.0
Max temperature: 60 °C
Carbon load: 9%
Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
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