HPLC Columns - Zorbax Columns
Agilent ZORBAX Eclipse Plus Column Specifications and Options
ZORBAX Eclipse Plus C8
Agilent ZORBAX Eclipse Plus C8 packing is made by chemically bonding a dense monolayer of dimethyl-n-octylsilane stationary phase to special Eclipse Plus silica. Eclipse Plus C8 columns can be used for acidic and neutral samples, and are well-suited for separations of basic compounds that produce poor peak shapes on other columns. Poroshell 120 EC-C8 is very similar to ZORBAX Eclipse Plus C18.
Particle Size (µm) Length (mm) ID Specifications Method Development Notes Working with LC/MS
1.8 µm particles (both RRHD, stable to 1200 bar, and RRHT, stable to 600 bar),
3.5 µm
5 µm
20 - 250 2.1
Custom options available
Pore size: 95Å
Surface area: 180 m2/g
pH: 2.0 - 9.0
Max temperature: 60 °C
Carbon load: 7%
Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
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