HPLC Columns - Zorbax Columns
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Agilent ZORBAX Eclipse Plus Column Specifications and Options
 
ZORBAX Eclipse Plus Phenyl-Hexyl
Agilent ZORBAX Eclipse Plus Phenyl-Hexyl packing is made by bonding a dense monolayer of dimethylphenylhexylsilane stationary phase to special Eclipse Plus silica. Eclipse Plus Phenyl-Hexyl columns provide alternate selectivity to C18 and C8.
 
Particle Size (µm) Length (mm) ID Specifications Applications Method Development Notes Working with LC/MS
1.8 µm particles (both RRHD, stable to 1200 bar, and RRHT, stable to 600 bar)
3.5 µm
5 µm
20 - 250 2.1
3.0
4.6
Prep
Custom options available
Double-endcapped
Pore size: 95Å
Surface area: 180 m2/g
pH: 2.0 - 8.0
Max temperature: 60 °C
Carbon load: 9%
Steroids, aromatic compounds with nitrogen or fluorine groups Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. This column can be used with 100% aqueous. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
 
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