HPLC Columns - Zorbax Columns
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ZORBAX Eclipse XDB Column Specifications and Options
 
ZORBAX Eclipse XDB-C18
The Agilent ZORBAX Eclipse XDB-C18 column is especially useful for the separation of acidic, basic and other highly polar compounds. It is made by first chemically bonding a dense monolayer of dimethyl-n-octadecylsilane stationary phase to special ZORBAX silica. The eXtra-Dense Bonding and exhaustive endcapping simultaneously deactivate the silica surface from deleterious interactions with samples, and also protect the silica support from dissolution in intermediate and higher pH environments.
 
Particle Size (µm) Length (mm) ID Specifications Method Development Notes Working with LC/MS
1.8 µm particles (both RRHD, stable to 1200 bar, and RRHT, stable to 600 bar
3.5 µm
5 µm
7 µm
20 - 250 2.1
3.0
4.6
Capillary and Prep
Custom options available
Double-endcapped
Pore size: 80Å
Surface area: 180 m2/g
pH: 2.0 - 9.0
Max temperature: 60 °C
Carbon load: 10%
Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
 
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