HPLC Columns - Zorbax Columns
ZORBAX Eclipse XDB Column Specifications and Options
ZORBAX Eclipse XDB-Phenyl
Agilent ZORBAX Eclipse XDB-Phenyl provides different selectivity from XDB-C18 and XDB-C8 and is an alternative to those phases for the separation of acidic, basic and other highly polar compounds. The eXtra-Dense Bonding and exhaustive endcapping simultaneously deactivate the silica surface from deleterious interactions with samples, and also protect the silica support from dissolution in intermediate and higher pH environments.
Particle Size (µm) Length (mm) ID Specifications Method Development Notes Working with LC/MS
1.8 µm particles (both RRHD, stable to 1200 bar, and RRHT, stable to 600 bar),
3.5 µm
5 µm
7 µm
20 - 250 1.0
Capillary and Prep
Custom options available
Pore size: 80Å
Surface area: 180 m2/g
pH: 2.0 - 9.0
Max temperature: 60 °C
Carbon load: 7.2%
Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. This column can be used with 100% aqueous. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
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