HPLC Columns - Zorbax Columns
Agilent ZORBAX StableBond Column Specifications and Options
ZORBAX StableBond SB-C3
ZORBAX SB-C3 is made by chemically bonding a sterically protected isopropyl stationary phase to a special ZORBAX silica. It is particularly well-suited for use with aggressive mobile phases (e.g. pH <2, high ionic strength, ion-pair additives, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases.
Particle Size (µm) Length (mm) ID Specifications Applications Method Development Notes Working with LC/MS
1.8 µm particles (both RRHD, stable to 1200 bar, and RRHT, stable to 600 bar)
3.5 µm
5 µm
7 µm
20 - 250 1.0
Capillary and Prep
Custom options available
Pore Size: 80Å
Surface area: 180 m2/g
pH: 1.0 - 8.0
Max temperature: 80 °C
Carbon load: 4%
ion-pair additives, high ionic strength compounds
Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. This column can be used with 100% aqueous. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
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