HPLC Columns - Zorbax Columns
Agilent ZORBAX StableBond Column Specifications and Options
ZORBAX StableBond SB-Phenyl
Agilent ZORBAX SB-Phenyl is made by chemically bonding a sterically protected phenyl stationary phase to special ZORBAX silica, designed to reduce or eliminate strong adsorption of basic compounds. The densely covered, sterically protected, diiosopropyl phenylsilane stationary phase is chemically stable and gives longer column life. ZORBAX SB-Phenyl is especially suited for MS applications.
Particle Size (µm) Length (mm) ID Specifications Method Development Notes Working with LC/MS
1.8 µm particles (both RRHD, stable to 1200 bar, and RRHT, stable to 600 bar)
3.5 µm
5 µm
7 µm
20 - 250 1.0
Capillary and Prep
Custom options available
Pore Size: 80Å
Surface area: 180 m2/g
pH: 1.0 - 8.0
Max temperature: 80 °C
Carbon load: 5.5%
Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. This column can be used with 100% aqueous. Use well-buffered mobile phases below pH 6. If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.
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