Agilent ZORBAX HPLC columns are available in a wide range of stationary phase chemistries to meet diverse separation requirements. Below are the major ZORBAX chemistries and their typical applications.
| Chemistry | USP Classification | Key Features | Typical Applications |
|---|---|---|---|
| Eclipse Plus C18 | L1 | Excellent peak shape, especially for basic compounds | Pharmaceutical assays, impurity profiling |
| Eclipse XDB-C18 | L1 | Extra Dense Bonding for broad pH compatibility | General-purpose HPLC methods |
| Eclipse XDB-C8 | L7 | Less hydrophobic than C18 | Faster analysis of moderately non-polar compounds |
| SB-C18 (StableBond) | L1 | Highly stable at low pH (1–8) | Acidic mobile phases, peptides, pharmaceuticals |
| SB-C8 | L7 | Low-pH stable, moderate retention | Drug analysis, routine QC |
| SB-Phenyl | L11 | π-π interactions improve aromatic selectivity | Aromatic compounds, isomers |
| Extend-C18 | L1 | Stable at high pH (up to ~11.5) | Basic compounds, high-pH separations |
| Bonus-RP | L60 | Embedded polar group allows 100% aqueous mobile phases | Polar pharmaceuticals, basic analytes |
| Eclipse Plus Phenyl-Hexyl | L11 | Enhanced aromatic selectivity | Aromatic drugs, PAHs, positional isomers |
| SB-CN (Cyano) | L10 | Reversed-phase and normal-phase capability | Polar compounds, method development |
| NH₂ (Amino) | L8 | Amino bonded phase | Carbohydrates, sugars, normal-phase separations |
| Carbohydrate | L8 | Dedicated carbohydrate separation | Sugars, carbohydrates, fosfomycin applications |
| ODS | L1 | Traditional octadecyl silica | General reverse-phase applications |
| 300SB-C18 | L1 | 300 Å pore size for large molecules | Peptides, proteins, biomolecules |
| 300SB-C8 | L7 | Large-pore C8 | Protein separations |
| 300Extend-C18 | L1 | High-pH stable large-pore column | Peptides under alkaline conditions |
| Rx-C18 | L1 | High-purity silica for reproducibility | Routine pharmaceutical analysis |
| Rx-SIL | L3 | Bare silica | Normal-phase chromatography |
Specialty ZORBAX Chemistries
- RRHT / RRHD Series – Rapid Resolution columns for UHPLC and high-speed separations.
- Poroshell 120 Series – Superficially porous particle technology for high efficiency with lower backpressure.
- Bio Series – Large-pore columns designed for proteins, peptides, and biopharmaceuticals.
How to Select the Right Chemistry
- General pharmaceutical analysis: Eclipse Plus C18, Eclipse XDB-C18
- Low-pH methods: StableBond (SB) series
- High-pH methods: Extend-C18
- Polar compounds: Bonus-RP
- Aromatic compounds: Phenyl-Hexyl or SB-Phenyl
- Proteins & peptides: 300SB series
- Carbohydrates & sugars: Carbohydrate or NH₂ columns
- Fast UHPLC analysis: RRHT/RRHD or Poroshell 120 series
Agilent’s ZORBAX portfolio covers virtually every HPLC separation challenge, from routine QC testing to advanced pharmaceutical, food, environmental, and biopharmaceutical analyses.
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